Echinaceaextract los yog EchinaceaPurpurea extractis siv los ua cov tshuaj muaj peev xwm tiv thaiv tsis zoo hauv North America thiab Europe thiab nws siv hauv cov tshuaj veterinary.
Kev tiv thaiv kev tiv thaiv thiab kev ua haujlwm
Echinacea yog siv los ua cov tshuaj tiv thaiv kev tiv thaiv ib txwm muaj hauv North America thiab Europe. Echinacea extract tsom pom muaj teeb meem loj los tiv thaiv kev cuam tshuam hauv glacial acetic acid-vim cov nas vascular permeability qauv, carrageenan-hia cov pab pawg nas o ua qauv, thiab LPS-vim cov nas macrophage TNF-re zais cov qauv. Stimal li al. kho cov nas uas muaj tus kab mob Candida albicans nrog Echinacea polysaccharides, thiab tom qab 24 teev, cov Candida hauv cov nas ob lub raum tau txo qis, qhia tias Echinacea tuaj yeem tiv thaiv cov kab mob Candida.
Antiviral thiab antitumor kev ua si
Binns SE li al. pom tias cov kev rho tawm ntawm Echinacea purpurea muaj qhov cuam tshuam rau herpes simplex type I virus. Wacker thiab Hilbig paub tseeb tias Echinacea extract muaj tus kab mob anti-influenza, tus kab mob pob sawv hlwv thiab tus kab mob vesicular stomatitis. Zhang Yingtao thiab lwm tus pom tias echinacea extract muaj kev sib txawv ntawm cov tshuaj tiv thaiv kab mob rau cov kab mob khaub thuas, tus kab mob Sakchi, thiab tus kab mob ua pa ntawm lub ntsws.
Hauv cov kab lis kev cai ntawm tes ntawm nas macrophages pretreated nrog echinacea, qog necrosis factor-α, IL-1, interferon-β2, thiab cytotoxicity tiv thaiv cov qog hlwb nce. Lub lim dej ntshiab polysaccharide cais tawm ntawm echinacea cell kab lis kev cai txhawb nqa kev ua haujlwm tsis siv neeg ntawm tib neeg leukocytes thiab nce kev muaj peev xwm ntawm cov hlwb tua Staphylococcus aureus.
Kev tshawb fawb txog Toxicology
Txog tam sim no, cov txheej txheem kev soj ntsuam tshuaj lom tshuaj lom neeg thiab kev sim tsiaj tau suav hais tias Echinacea yog kiag li tsis muaj tshuaj lom. Lub tuam txhab tau cog lus rau Shanghai Kev Tshawb Fawb Kev Tshawb Nrhiav Kev Tshawb Fawb los kawm txog kev cuam tshuam ntawm echinacea rau nas hemolysin, phagocytic kev ua haujlwm ntawm peritoneal macrophages thiab NK cell cell. Cov txiaj ntsig pom tau hais tias echinacea tuaj yeem txhim kho qhov tsis muaj kev tiv thaiv tsis meej, kev ua kom lub siab thiab kev tiv thaiv kab mob ntawm cov tsiaj.
(1) Qhov cuam tshuam ntawm Echinacea purpurea rau hemolysin hauv cov nas
Tsib caug Kunming nas tau muab faib ua 5 pawg. Echinacea siab, nruab nrab, thiab qis koob tshuaj pawg tau muab tshuaj intragastrically ntawm koob tshuaj ntawm 2.25 g / kg, 0.75 g / kg, thiab 0.25 g / kg feem. Kev tswj cov tshuaj tiv thaiv kab mob siab-polysaccharide tau muab rau cov tshuaj ntawm qhov muaj peev xwm yog 0.1 g / kg, ib hnub ib zaug rau 2 lub lis piam. 4 hnub ua ntej ntawm txoj kev tswj hwm, txhua tus nas tau tso dej txhaj tshuaj nrog 0.2 ml ntawm 20% yaj liab ntshav ntshav ncua. Tom qab 4 hnub, cov ntshav tau coj los ntawm lub qhov muag, cov ntshav tau sib cais, thiab diluted 500 zaug, 10% 0.5 ml ntawm cov ntshav liab cov ntshav liab tau ntxiv rau 1 ml ntawm diluted cov ntshav, thiab 1 ml ntawm pretreated 1:10 guinea npua ntshav tau ntxiv rau, thiab tom qab ntawd muab tso rau hauv chav dej da dej ntawm 37 ° C rau 10 feeb Cov dej tso tseg cov tshuaj tiv thaiv. Centrifuge rau 10 ml ntawm 2000 rpm, noj 1 ml ntawm supernatant thiab ntxiv 3 ml ntawm Duchenne' s reagent, sawv ntsug li 10 feeb, thiab ntsuas OD tus nqi ntawm lub yoj ntawm 540 nm.
Peb pawg pawg ntawm Echinacea tuaj yeem ua rau kom qhov txiaj ntsig ntawm hemolysin ntawm cov nas nyob rau hauv cov tshuaj tiv thaiv kev noj tshuaj, qhia tias nws muaj cov txiaj ntsig tseem ceeb hauv kev tsim cov nas hemolysin antibody, qhia tias qhov tshuaj muaj peev xwm txhim kho kev tiv thaiv kev tiv thaiv humoral.
(2) Qhov cuam tshuam ntawm Echinacea purpurea rau ntawm kev ua haujlwm phagocytic ntawm nas peritoneal macrophages
Cov pab pawg sim thiab muab tshuaj kho tau zoo ib yam li saum toj no. Cov nas tau txhaj tshuaj tso ntshav nrog 2% nqaij qaib ntshav liab 2ml / nas, 4 teev tom qab, cov nas tau raug muab tua, lub plab zom mov tau raug ntxuav nrog cov dej qab ntsev, cov kua dej ntxuav tau sau, centrifuged, supernatant tau nchuav, thiab lub cell smear tau sau. Tom qab ziab, zas xim nrog Wright' s staining tov thiab soj ntsuam hauv qab roj tshuab. Suav cov qe ntshav liab tau nqos 100 macrophages, thiab suav los ntawm cov mis hauv qab no:
Feem pua ntawm phagocytosis=tus lej ntawm macrophages phagocytosing nqaij qaib ntshav liab / tus lej ntawm 100 macrophages
Phagocytosis Performance index=tus naj npawb ntawm cov nqaij qaib ntshav liab engulfed / tus naj npawb ntawm macrophages hauv 100
Qhov peb pawg tshuaj ntawm Echinacea tuaj yeem nce qhov feem pua ntawm phagocytosis ntawm nas peritoneal macrophages nyob rau hauv ib qho kev txhaj tshuaj raws.
(3)Kev cuam tshuam ntawm Echinacea purpurea ntawm kev ua si ntawm NK hlwb hauv C57BL / 6J nas
Cov pab pawg thiab kev noj tshuaj tau zoo ib yam li saum toj no, caj dab raug txiav tawm thiab cov nas tau raug fij tseg. Tus spleens tau coj los ua qhov qub, tus splenic lymphocytes tau sib cais, thiab cov haujlwm ntawm NK hlwb tau txiav txim siab.
Nws tuaj yeem pom los ntawm kev sim tias qhov kev xav ntau pawg Echinacea purpurea pawg tuaj yeem txhim kho kev ua si ntawm nas NK hlwb.
cov kev ua tau zoo mob los ntawm echinacea
Lub koom haum ntawm Suav Veterinary Tshuaj ntawm Nanjing Agricultural University tau ua qhov kev sim tshuaj tiv thaiv-ua kom zoo dua ntawm echinacea hmoov tiv thaiv cov tshuaj tiv thaiv khov-qhuav ntawm Newcastle kab mob. 1-hnub-laus qe qaib tau muab faib ua 7 pawg thaum tsa 11-hnub-laus. Cov kev qhia tshwj xeeb hauv pawg thiab kev kho mob muaj nyob hauv Daim Ntawv 4.
(1) Kev hloov pauv hloov pauv ntawm Newcastle kab mob antibody titer
Nws tuaj yeem pom tau tias thaum hnub nyoog 21, 28, 35 thiab 42 hnub, cov tshuaj tiv thaiv qib hauv pawg siab Echinacea koob tshuaj thiab pawg Echinacea nruab nrab koob tau nce siab dua li ntawm cov tshuaj tswj hwm thiab kev tswj hwm dawb paug. pab pawg.
(2) Kev hloov pauv ntawm lymphocyte proliferation
Thaum muaj hnub nyoog 21, 35, thiab 42 hnub, A570 tus nqi ntawm Echinacea siab thiab nruab nrab koob pawg neeg tau nce siab dua li lwm peb pawg (P< 0.05),="" thiab="" tsis="" muaj="" qhov="" sib="" txawv="" ntawm="" ob="" pawg="" (p=""> 0.05); pawg Echinacea qis pawg thiab Cov pab pawg tswj hwm tshuaj tau nce siab siab dua pawg tshuaj tiv thaiv (P<>
(3) Qhov cuam tshuam ntawm kev tiv thaiv kev tiv thaiv cov tshuaj lom thiab tiv thaiv kev tiv thaiv
Txog hnub thib 3 tom qab qhov kev sib tw, tshwj tsis yog rau lub siab cov tshuaj ua kua echinacea hmoov, cov qaib ntawm txhua pawg tau pib qhia cov nyom ntsuab xoob xoob, tab sis lawv cov ntsuj plig thiab kev noj haus tau ib txwm; Hnub 4, tus qaib ntawd pom tias muaj kev nyuaj siab thiab noj zaub mov tsawg. Echinacea hmoov pawg loj tshaj plaws tsis muaj kab mob, Echinacea hmoov-nruab nrab koob pawg muaj qhov tshwm sim qis tshaj, thiab Echinacea hmoov siab-koob tshuaj thiab nruab nrab koob muaj cov xwm txheej loj dua li peb pawg.
Txog hnub 5, kev tuag ploj mus pib tshwm sim hauv pawg sib tw tswj hwm, pawg tswj hwm tshuaj thiab pab pawg qis echinacea, thiab kev tuag nres rau hnub 10. Tsis muaj kev tuag hauv cov pawg siab thiab nruab nrab ntawm Echinacea, thaum cov neeg tuag ntawm lwm peb pawg tau nce siab.
Txog hnub 14, txhua tus qaib tseem muaj sia rov qab los rau kev noj qab haus huv. Kev tiv thaiv kab mob tiv thaiv ntawm cov qib siab thiab nruab nrab koob pawg ntawm Echinacea purpurea tau mus txog 100%, uas tau nce siab tshaj li lwm peb pawg.
Hloov pauv kev loj hlob zoo
Ua ntej qhov kev sib tw (14 txog 42 hnub), tsis muaj qhov sib txawv ntawm qhov nruab nrab ntawm qhov hnyav txhua hnub, kev pub noj, thiab kev sib npaug ntawm qhov hnyav ntawm txhua pawg. Tom qab kev sib tw (43-56 hnub), pawg tshuaj tiv thaiv zoo tshaj plaws yog cov tshuaj tiv thaiv uas tsis muaj kev sib tw. Piv nrog txhua pab pawg sib tw, qhov nce nruab nrab txhua hnub kev hnyav ntawm Echinacea pawg pawg siab tshaj yog qhov siab tshaj plaws, ua raws Echinacea hmoov-nruab nrab pawg; qhov pub-rau-qhov sib piv yog qhov siab tshaj plaws hauv pawg sib tw thiab tau nce siab dua li lwm pawg sib tw, thiab Echinacea koob tshuaj pab pawg tau qis tshaj. Cov.
Kev ntsuas thiab kev siv thiab ntau npaum li cas
Kev Taw Qhia: Siv ua ntej thiab tom qab txhaj tshuaj tiv thaiv kab mob los ntawm npua thiab qaib, nws tuaj yeem nce cov tshuaj tiv thaiv titer thiab ncua lub sijhawm tiv thaiv cov tshuaj tiv thaiv; siv rau cov kab mob khaub thuas xws li mob khaub thuas ua npaws, mob atypical Newcastle kab mob, kis mob bursitis; npua pob ntseg xiav, kab mob Adjuvant los kho cov mob cyclovirus, thiab lwm yam.
Kev siv thiab ntau npaum li cas: ntxiv 1000 grams echinacea hmoov sib tov ib tuj ntawm cov khoom noj; ntxiv 1000 ml ntawm echinacea tov ib tuj dej rau 5-7 hnub.
